Characterization of human intestinal bifidobacteria using competitive PCR and PCR-TTGE
نویسندگان
چکیده
منابع مشابه
Quantitative PCR with 16S rRNA-gene-targeted species-specific primers for analysis of human intestinal bifidobacteria.
A highly sensitive quantitative PCR detection method has been developed and applied to the distribution analysis of human intestinal bifidobacteria by combining real-time PCR with Bifidobacterium genus- and species-specific primers. Real-time PCR detection of serially diluted DNA extracted from cultured bifidobacteria was linear for cell counts ranging from 10(6) to 10 cells per PCR assay. It w...
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ژورنال
عنوان ژورنال: FEMS Microbiology Ecology
سال: 2006
ISSN: 0168-6496,1574-6941
DOI: 10.1111/j.1574-6941.2005.00005.x